TY - JOUR ID - 15313 TI - Oxidative DNA Damage and Pro-inflammatory Response In Chronic Exposure To Cement Dust JO - Asia Pacific Journal of Medical Toxicology JA - APJMT LA - en SN - 2322-2611 AU - Obaji-Ogar, Lara Taiye AU - Nsonwu-Anyanwu, Augusta Chinyere AU - Odum, Friday Acho AD - Department of Medical Laboratory Science,University of Calabar, Nigeria AD - University of Calabar, Nigeria AD - Department of Medical Laboratory Science, University of Calabar, Nigeria Y1 - 2020 PY - 2020 VL - 9 IS - 1 SP - 3 EP - 10 KW - cement KW - Heavy metals KW - Inflammation Oxidative Stress DO - 10.22038/apjmt.2020.15313 N2 - Background: Inflammatory cell activation, oxidative stress and oxidative DNA damage have been associated with exposure to cement dust. Biomarkers of oxidative stress, oxidative DNA damage, inflammation and heavy metals were estimated in cement loaders. Methods: Ninety men (45 cement loaders and 45 controls) were recruited into this comparative cross-sectional study. Total antioxidant capacity (TAC), total plasma peroxides (TPP), malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO) and uric acid (UA) were estimated by colorimetry, arsenic (As), chromium (Cr) and cadmium (Cd) by atomic absorption spectrophotometry and tumor necrosis factor alpha (TNF-α), 8-hydroxy-2-deoxyguanosine (8-OHdG) by enzyme linked immunosorbent assay.  Results: Cement loaders had increased lipid peroxidation (MDA, TPP, OSI), inflammation (TNF-ɑ) and heavy metals (As, Cr) and lower antioxidants (UA, TAC, GSH) compared to controls (p<0.05). Increasing duration of exposure to cement dust was associated with higher lipid peroxidation, Cd, TNF-α and oxidative DNA damage (8-OHdG) (p<0.05). Negative correlation was observed between TAC and duration of exposure (r=-0.375, p=0.011) and positive correlations between TPP and duration of exposure (r=0.614, p=0.000), TNF-α and 8-OHdG (r=0.492, p=0.001) in cement loaders.  Conclusion: Chronic exposure to cement dust is associated with depletion of antioxidants, increased lipid peroxidation, oxidative stress, inflammation and oxidative DNA damage. These may be implicated in the development of chronic lung conditions. UR - https://apjmt.mums.ac.ir/article_15313.html L1 - https://apjmt.mums.ac.ir/article_15313_4c78440dfb269187c5d7a86b30a8af22.pdf ER -