Co-Administration of Vitamin E and Homtamin Ginseng Prevented and Reversed Sodium Fluoride-Induced Testicular Dysfunction Through Inhibition of Oxidative Influx and Hormonal Modulation Mechanism in Wistar Rats

Oyovwi, Mega Obukohwo

doi:10.22038/apjmt.2024.76819.1440

Document Type : Original Article

Author

Mega Obukohwo Oyovwi

Department of Physiology, Faculty of Basic Medical Science, Adeleke University, Ede, Osun State, Nigeria.

https://doi.org/10.22038/apjmt.2024.76819.1440

Abstract

Background: Reproductive damage from sodium fluoride exposure is a concern, but there's limited scientific data on the synergistic effect of Homtamin ginseng and vitamin E. A potential remedy could include Homtamin ginseng and vitamin E supplementation, but further research is needed. This research was conducted to investigate the effects of Homtamin ginseng and vitamin E on the reproductive damage induced by sodium fluoride exposure. Different treatment protocols were employed, such as administering drugs alone, preventive measures, and reversal approaches.
Methods: In the drug alone or preventive-protocol, rats received oral treatment of Saline (2 mL/kg), Homtamin ginseng (40 mg/kg/day), vitamin E (150 mg/kg/day) or both alone repeatedly for 28 days, or in combination with sodium fluoride (100 ppm/kg/p.o./day) from days 15-28. In the reversal-protocol, the animals received NaF for 28 days prior to saline, Homtamin ginseng, vitamin E or Homtamin ginseng + vitamin E from days 15-28. Results: The result showed a (P < 0.05) increase in testicular malondialdehyde and a decrease in the activities of testicular superoxide dismutase, catalase and glutathione peroxidase in the group exposed to sodium fluoride. These changes were significantly (P < 0.05) ameliorated by vitamin E and Homtamin ginseng. More so, sodium fluoride altered testicular and brain weight, spermatogenesis, hormonal profile, fertility test as well as testicular architecture negatively following treatment with sodium fluoride. However, Homtamin ginseng and vitamin E or combination of both prevented and reversed the effects of sodium fluoride on spermatogenesis, hormonal profile, fertility and mating indices in rats.
Conclusion: In conclusion, Homtamin ginseng and vitamin E prevented and reversed sodium fluoride-induced testicular dysfunction through inhibition of oxidative influx and hormonal modulation mechanism in Wistar rats.

Keywords

Main Subjects

Toxicology

References

1. Abbasnia, A., Alimohammadi, M., Mahvi, A. H., Nabizadeh, R., Yousefi, M., Mohammadi, A. et al. Assessment of groundwater quality and evaluation of scaling and corrosiveness potential of drinking water samples in villages of Chabahr city, Sistan and Baluchistan province in Iran. Data in Brief, 2018; 16, 182–192. https://doi.org/10.1016/j.dib.2017.11.003
2. Dehghani, M. H., Zarei, A., Yousefi, M., Baghal Asghari, F., & Haghighat, G. A. Fluoride contamination in groundwater resources in the southern Iran and its related human health risks. DESALINATION AND WATER TREATMENT, 2019; 153, 95–104. https://doi.org/10.5004/dwt.2019.23993
3. Li, P., He, X., Li, Y., & Xiang, G. Occurrence and Health Implication of Fluoride in Groundwater of Loess Aquifer in the Chinese Loess Plateau: A Case Study of Tongchuan, Northwest China. Expo Health, 2018; 11(2), 95–107. https://doi.org/10.1007/s12403-018-0278-x
4. He, X., Li, P., Wu, J., Wei, M., Ren, X., & Wang, D. Poor groundwater quality and high potential health risks in the Datong Basin, northern China: research from published data. Environ Geochem Health, 2020; 43(2), 791–812. https://doi.org/10.1007/s10653-020-00520-7
5. Adimalla, N., & Li, P. Occurrence, health risks, and geochemical mechanisms of fluoride and nitrate in groundwater of the rock-dominant semi-arid region, Telangana State, India. Human and Ecological Risk Assessment: An. Int. Journal, 2018; 25(1–2), 81–103. https://doi.org/10.1080/10807039.2018.1480353
6. Mohammadi, A. A., Yousefi, M., & Mahvi, A. H. Fluoride concentration level in rural area in Poldasht city and daily fluoride intake based on drinking water consumption with temperature. Data in Brief, 2017; 13, 312–315. https://doi.org/10.1016/j.dib.2017.05.045
7. Grant, R. Detecting, treating, controlling and preventing tuberculosis: developing the NICE guidelines. Primary Health Care, 2008; 18(2), 30–33. https://doi.org/10.7748/phc2008.03.18.2.30.c6511
8. James, P., Harding, M., Beecher, T., Browne, D., Cronin, M., Guiney, H., et al. Impact of Reducing Water Fluoride on Dental Caries and Fluorosis. J Dent Res., 2020; 100(5), 507–514. https://doi.org/10.1177/0022034520978777
9. Giwa, A. S., Memon, A. G., Ahmad, J., Ismail, T., Abbasi, S. A., Kamran, K., et al. Assessment of high fluoride in water sources and endemic fluorosis in the North-Eastern communities of Gombe State, Nigeria. Poll. Bio., 2021; 33(1), 31–40. https://doi.org/10.1080/26395940.2021.1908849
10. Zuo H, Chen L, Kong M, Qiu L, Lü P, Wu P, Yang Y, Chen K. Toxic effects of fluoride on organisms. Life sciences. 2018 Apr 1;198:18-24.
11. Żwierełło W, Maruszewska A, Skórka-Majewicz M, Gutowska I. Fluoride in the Central Nervous System and Its Potential Influence on the Development and Invasiveness of Brain Tumours—A Research Hypothesis. International Journal of Molecular Sciences. 2023 Jan 13;24(2):1558.
12. Azhdarpoor, A., Radfard, M., Rahmatinia, M., Hashemi, H., Hashemzadeh, B., Nabavi, S et al. Data on health risk assessment of fluoride in drinking water in the Khash city of Sistan and Baluchistan province, Iran. Data in Brief, 2018; 21, 1508–1513. https://doi.org/10.1016/j.dib.2018.08.139
13. Stangvaltaite-Mouhat, L., Puriene, A., Stankeviciene, I., & Aleksejuniene, J. Fluoride in the drinking water and dental caries experience by tooth surface susceptibility among adults. BMC Oral Health, 2021; 21(1). https://doi.org/10.1186/s12903-021-01598-w
14. Zhao H, Zhu Y, Zhao Y, Wang T, Li H, Yang J, Cheng X, Wang J, Wang J. Alleviating effects of selenium on fluoride-induced testosterone synthesis disorder and reproduction toxicity in rats. Ecotoxicology and Environmental Safety. 2022 Dec 1;247:114249.
15. Yousefi M, Mohammadi AA, Yaseri M, Mahvi AH. Epidemiology of drinking water fluoride and its contribution to fertility, infertility, and abortion: an ecological study in West Azerbaijan Province, Poldasht County, Iran. Fluoride. 2017 Jul 1;50(3):343-53.
16. Gulegoda CR, Dissanayake CB, Amarasekara DS, Wijeratne S, Premadasa JK, Chandrajith R, Udagama PV. Impact of fluoride exposure on male reproductive parameters: a pilot case–control study in Sri Lanka. Exposure and Health. 2022 Jun;14(2):447-57.
1. Emojevwe, V., Naiho, A. O., Nwangwa, E. K., Oyovwi, M. O., Anachuna, K. K., Agbanifo-Chijiokwu, E., et al. Duration-dependent effects of high dose of phthalate exposure on semen quality in adult male rats. JBRA assist. Reprod, 2021; https://doi.org/10.5935/1518-0557.20210033
2. Adelakun SA, Ukwenya VO, Ojewale AO, Aniah JA, Kolawole BP. Excessive exposure to sodium fluoride impaired spermatogenesis, induced hormonal and biochemical imbalance and testicular atrophy: Ameliorating potential of bioactive component of Solanum aethiopicum supplementation. Phytomedicine Plus. 2023 Aug 1;3(3):100458.
3. Moghaddam, V. K., Yousefi, M., Khosravi, A., Yaseri, M., Mahvi, A. H., Hadei, M., Mohammadi, A. Aet al. High Concentration of Fluoride Can Be Increased Risk of Abortion. Trace Ele. Res., 2018; 185(2), 262–265. https://doi.org/10.1007/s12011-018-1250-0
4. Bittencourt LO, Dionizio A, Ferreira MK, Aragão WA, de Carvalho Cartágenes S, Puty B, do Socorro Ferraz Maia C, Zohoori FV, Buzalaf MA, Lima RR. Prolonged exposure to high fluoride levels during adolescence to adulthood elicits molecular, morphological, and functional impairments in the hippocampus. Scientific Reports. 2023 Jul 8;13(1):11083.
5. Mesram, Napgapuri, K., Banala, RR., Nalagoni, CR., Pratap, N., Karnati P.R. Quercetin treatment against NaF induced oxidative stress related neuronal and learning changes in developing rats. J. King Saud Uni- Sci, 2017; 29( 2):221-229
6. Sukmawati, Y., Arisanty, D., Tofrizal, A., & Amir, A. Vitamin E ameliorates testicular histological features and androgen binding protein levels in testicle of rats induced by allethrin. J Adv Vet Anim Res., 2019; 6(4), 486–491. https://doi.org/10.5455/javar.2019.f372.
7. Shittu ST, Oyeyemi ,WA, Okewumi TA, Salman TM. Role of oxidative stress in therapeutic administration of artesunate on sperm quality and testosterone levels in male albino rats. Afr J Biotechnol, 2013; 12(1):70–3. https://doi.org/10.5897/AJB12.315.
8. Kunle-Alabi OT, Akindele OO, Oyowvi MO, Duro-Ladipo M and Raji Y. Effects of coconut water on reproductive functions in danazol treated Wistar rats, 2015; FASEB J. 29(1); 685.
9. Burses J, Bureseva O, Huston JP. Techniques and basic experiments for the study of brain and behavior. New York: Elsevier Science Publishers. 1983, 117-121.
10. Ilodigwe EE, Igbokwe EN, Ajaghaku DL, Ihekwereme CP. Aphrodisiac activity of ethanol root extract and fractions of Landolphia Dulcis (Sabine) Pichon. International Journal of Pharmaceutical Sciences and Research. 2013 Feb 1;4(2):813.
11. Woode, E., Alhassan, A., Abaidoo, C.S. Effect of ethanolic fruit extract of Xylopia aethiopica on reproductive function of male rats. Int. J. Pharm. Biomed. Res, 2011; 2, 161.
12. Naiho, A. O., Ekene, E. N., Ebeye, M. O., Olowe, G. T., & Odigie, M. O. Cadmium Chloride Reduces Testicular and Epididymal Weights with Degenerative Histoarchitectural Changes in Testis and Pituitary Gland of Wistar Rats. J. Ap. Life Sci. Int., 2018; 1-7.
13. Ullah, R., Zafar, M. S., & Shahani, N. Potential fluoride toxicity from oral medicaments: A review. Iran J Basic Med Sci., 2017; 20(8), 841–848. https://doi.org/10.22038/IJBMS.2017.9104
14. Peckham, S., & Awofeso, N. Water fluoridation: a critical review of the physiological effects of ingested fluoride as a public health intervention. TheScientificWorldJournal, 2014; 293019. https://doi.org/10.1155/2014/293019
15. Al-tai FT. Histological Study of The Effects of Sodium Fluoride and The Protective Role of Vitamin E on The Testis of Albino Rats. Eastern Journal of Medicine. 2023 Oct 1;28(4).32. Sm S, Mahaboob Basha P. Fluoride exposure aggravates the testicular damage and sperm quality in diabetic mice: protective role of ginseng and banaba. Biological Trace Element Research. 2017 Jun;177:331-44.
16. Al-Attar A.M. Antioxidant effect of vitamin E treatment on some heavy metals-induced renal and testicular injuries in male mice. Saudi J. Biol. Sci, 2011; 18(1):63–72.
17. Bassey I.E., Gali R.M., Udoh A.E. Fertility hormones and vitamin E in active and passive adult male smokers in Calabar, Nigeria. PLoS One, 2018; 13(11) e0206504-e0206504.
18. Miltonprabu, S., & Thangapandiyan, S. Protective effect of epigallocatechin gallate on fl-induced oxidative stress related haematotoxicity in rats. Toxicol. Rep,2013; 1, 2.
19. Hong F, Zheng C, Xu DG, Qian YL. Chronic combined effects of fluoride and arsenite on the Runx2 and downstream related factors of bone metabolism in rats. Zhonghua Yu Fang Yi Xue Za Zhi., 2013; 47: 794-798.37.
20. Jia YF, Feng Q, Ge ZY, Guo Y, Zhou F, Zhang KS, Wang XW, Lu WH, Liang XW, Gu YQ. Obesity impairs male fertility through long-term effects on spermatogenesis. BMC urology. 2018 Dec;18(1):1-8.
21. Thangapandiyan, S., & Miltonprabu, S. Epigallocatechin gallate supplementation protects against renal injury induced by fluoride intoxication in rats: Role of Nrf2/HO-1 signaling. Toxicol Rep., 2014; 1, 12–30. https://doi.org/10.1016/j.toxrep.2014.01.002
22. 39. Romanello, V., & Sandri, M. Mitochondrial Quality Control and Muscle Mass Maintenance. Front physiol., 2016; 6, 422. https://doi.org/10.3389/fphys.2015.00422
23. Oehniger, S., Franken, D.R., Ombelet, W. Sperm functional test. Fertil Steril. 2014; 102, 1528–1533.
24. Oyovwi, M. O., Ben-Azu, B., Edesiri, T. P., Victor, E., Rotu, R. A., Ozegbe, Q. E., et al Kolaviron abates busulfan-induced episodic memory deficit and testicular dysfunction in rats: The implications for neuroendopathobiological changes during chemotherapy. Biomed. Pharmaco., 2021; 142, 112022. https://doi.org/10.1016/j.biopha.2021.112022
25. Oeniger, S. Clinical management of male infertility in assisted reproducrion: ICSI and beyond. Int. J. Androl., 2011, 34, e319–e329.
26. Elghareeb MM, Elshopakey GE, Rezk S, Ateya A, El-Ashry ES, Shukry M, Ghamry HI, Alotaibi BS, Hashem NM. Nigella sativa oil restores hormonal levels, and endocrine signals among thyroid, ovarian, and uterine tissues of female Wistar rats following sodium fluoride toxicity. Biomedicine & Pharmacotherapy. 2024 Jan 1;170:116080.44.
27. Pal P, De A, Roychowdhury T, Mukhopadhyay PK. Vitamin C and E supplementation can ameliorate NaF mediated testicular and spermatozoal DNA damages in adult Wistar rats. Biomarkers. 2022 May 19;27(4):361-74.45.
28. Tang W, Xiao Y, Long Y, Li Y, Peng F, Zhu C, He T, Lou D, Zhu Y. Sodium fluoride causes oxidative damage to silkworm (Bombyx mori) testis by affecting the oxidative phosphorylation pathway. Ecotoxicology and Environmental Safety. 2021 Jul 15;218:112229.
29. Isaac A. Adedara, Bolanle F. Olabiyi, TeminiJesu D. Ojuade, Umar F. Idris, Esther M. Onibiyo, and Ebenezer O. Farombi. Taurine reverses sodium fluoride-mediated increase in inflammation, caspase-3 activity, and oxidative damage along the brain–pituitary–gonadal axis in male rats. Can J Physiol Pharmacol, 2017; 95(9): 1019-1029.

Asia Pacific Journal of Medical Toxicology

Co-Administration of Vitamin E and Homtamin Ginseng Prevented and Reversed Sodium Fluoride-Induced Testicular Dysfunction Through Inhibition of Oxidative Influx and Hormonal Modulation Mechanism in Wistar Rats

References

References

Volume 12, Issue 4 - Serial Number 4
December 2023
Pages 123-136

Co-Administration of Vitamin E and Homtamin Ginseng Prevented and Reversed Sodium Fluoride-Induced Testicular Dysfunction Through Inhibition of Oxidative Influx and Hormonal Modulation Mechanism in Wistar Rats

References

References

Volume 12, Issue 4 - Serial Number 4December 2023Pages 123-136

Volume 12, Issue 4 - Serial Number 4
December 2023
Pages 123-136