Document Type : Original Article


Senior Lecturer Gr II, Faculty of Siddha Medicine, Trincomalee Campus, Eastern University, Sri Lanka


Background: Nonmetallic and mild toxic Sulphur is abundantly available in world. Kanthakam is the name for Sulphur in Siddha medicine. Sulphur is one of unavoidable substance for medicine preparations with deferent purification methods. According to Siddha concept of Purification (Shuddhi) not only a process of purification or detoxification, but also process to enhance potency and efficacy of the medicine.

Methods: The Unpurified Sulphur (S1) and Purified Sulphur Method I (S2), Method II (S3), Method III (S4) and Method IV(S5) Samples were employed for Comparative analysis of organoleptic character, Solubility profile, loss on drying, total and acid insoluble ash, alcohol and water soluble extractive, pH, Sulphur concentration, Microbial test, Aflatoxin assay by TLC, Test for basic and acid radicles, FTIR, and SEM

Results: finding gave considerable changes between unpurified Sulphur (S1) and purified samples. The purity percentage of Sulphur, were found increased from 8.97% w/w to 59.04% w/w and the particle size were reduces from 1247 d.nm to 468.0 d.nm in the S5 sample. FTIR show the functional group changes from C=C- alkene and C-I - Alkyl & Aryl Halides in to O-H-Alcohol, N-H- amine salt, C-H- alkane, C=C- alkene and C=O Esters & δ-lactone. Purified Sulphur samples show changes in Sulphur content, Particle size, functional group and solubility were within the safety limits as FDA permissible quantity. Heavy metals was not found in any samples.

Conclusion: According to comparative analysis, it is vital to select purifying methods for preparing medicines in accordance with the text.


Main Subjects

  1. Thiyagarajan Gunapadam- thathu jeeva vaguppu part 2&3. Chennai 600106 : Department of indian medicine and homeopathy; 2009.  pp. 302- 320.
  2. Earnshaw A, Green wood NN. Chemistry of the elements.2nd ed; 1997.
  3. Agasthiyar kanma sooththiram. vol. 3rd edition; 1988. p. 35.
  4. Gurusironmani Pon, Siddha toxicology: indian medicine and homeopathy chennai, 1999. p. 4.
  5. Ahmad mushtaq. In which organic solvent is suitable for sulfur?.(internet) 2015: retrieved from:,.
  6. Sulfurs, 2020. available from: https:///#:~:text=sulfur particularly in its s, practically insolubleinall solvents. [journal]. -
  7. Schwefel teil K, lieferung T, weinheim VCH, Bamberg M. Gmelin handbuch der anorganischen chemie, , dissertation, univ. Saarbrücken, 1959. [book section]. - 1953.
  8. Agiba ahmed. What is the limit for moisture content (% loss in drying)?.   2017. Retrieved from: [book section].
  9. Kim D, Kim B, Yun E, Statistical quality control of total ash, acid-insoluble ash, loss on drying, and hazardous heavy metals contained in the component medicinal herbs of “ssanghwatang” [book section]. - [s.l.] : , a widely used oriental formula in korea. J nat med 67, , 2013. - vols. Https://
  10. Anonymous, Pharmacopoeial standards for ayurvedic formulations. New delhi: central council for research in ayurveda and siddha, [report]. - [s.l.] : ministry of health and family welfare; , 1987.
  11. Mathew KM, the flora of the palni hills, south india. Part 2.: the rapinat herbarium, st.joseps college, tiruchchirapalli, india., 1990.
  12. Kimberley Tang Vikrama Baskaran and Mehdi Nemati bacteria of the sulphur cycle: an overview of microbiology, biokinetics and their role in petroleum and mining industries, : ,biochemical engineering journal 44(1):, april 2009. - pp. 73-94.
  13. Tamura K, Stecher G, Peterson D, Filipski A and Kumar S, Molecular evolutionary genetics analysis):): version 6.0. Mol biol evol., 2013. - pp. 2725-9.
  14. Pedersen K, exploration of deep intraterrestrial microbial life: current perspectives. Fems microbiol. Lett. 2000. - vols. 185:9-16.
  15. Xu hb tsukuda M, Takahara Y, Sato T, Gu Jd, Katayama Y, lithoautotrophical oxidation of elemental sulfur by fungi including fusarium solani isolated from sandstone angkor temples, int. Biodeter. Biodegrd. 2018. - vols. 126:95-102.
  16. Bankole SA. Adebanjo A mycotoxins in food in west africa:: current situation and possibilities of controlling it. Afr j biotechnol 2: ., 2003. - pp. 254-263.
  17. Rajalakshmy MR, Sruthi CV, Maniyan AK,  Vendamirtham S, Neeraj PT and  Sindhu A, physicochemical transformation of sulfur during pharmaceutical processing in traditional indian medicine: indian journal of pharmaceutical science, 2017.
  18. Testa B, In comprehensive medicinal chemistry ii, : elsevier science, 2007. - 978-0-08-045044-5.
  19. Manyangadzea M, Chikuruwo NHM, Narsaiah TB, Radhakumari M, Danha G, enhancing adsorption capacity of nano-adsorbents via surface modification:: south african journal of chemical engineering, 2020. - vol. 31.
  20. Murphy CJ,. "Materials science: nanocubes and nanoboxes". Science. 298 (5601): 13 december 2002. - pp. 2139–2141. - doi:10.1126/science.1080007. Pmid 12481122. S2cid 136913833.
  21. Madhavan, Agasthiyar charakku suddi,: tamil university thanjavur,, 2009.
  22. Muthaliyar murugesa Nanjumurivu nool,: indian medicine and homeopathy chennai; edition, 2003. - pp. 6-7.
  23. Ramasamy PR, siddha maruthuvathil kanthakathin payan padukal: pirarthan velidakam, 115, pethaya road, rajapalayam, 2016. - vol. Siddha maruthuva kazhanjiyam part 5.