Document Type : Original Article
Authors
- Sadaf Saeedi 1
- Ali Olfati 2
- Tayebeh Sadeghi 3
- Firoozeh Veisi 4
- Maryam Zanganeh 4
- Nasrin Jalilian 4
- Sareh Farshadfar 5
- Samad Nazemi 6
- Hamed Khorami 7
1 Department of Pathobiology, Faculty of veterinary sciences, science and Research branch, Islamic Azad university, Tehran, Iran
2 Clinical Research Development Center, Motazedi Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran
3 Department of Physiology, Faculty of Medicine, Kerman Branch, Islamic Azad University, Kerman, Iran
4 Department Obstetrics & Gynecology, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran
5 Department of Pediatrics, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
6 Cellular and Molecular Research Center, Department of Physiology and Pharmacology, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
7 Department of Medical Chemistry, Ghadr University of Kuchesfahan, Gilan, Iran
Abstract
Background: Arsenic (AS) is widely distributed in our surroundings, causing various health problems like neurological disorders. The current research was designed to investigate the effect of the anti-oxidant and anti-inflammatory content of sake yeast on the recovery of brain damage in an AS-treated rat's model with behavioral, oxidative stress, and immunogenetics assessment.
Method: Twenty-four male rats were treated with AS (3 mg/kg b.wt. per day) alone or in combination form with sake (45 mg/kg b.wt. per day), and animals received them for 30 days in drinking water (n=6/group). The initial mechanism of action was explored by behavioral tests (rotarod, amphetamine rotation, and spatial memory(, oxidative assay, and histopathology methods.
Results: Considering the vehicle group, induction of brain abnormalities by AS significantly (P<0.05) decreased the number of substantia nigra neurons, total antioxidant capacity, glutathione peroxidase activity and increased the amount of α-synuclein protein and led to the massive accumulation of malondialdehyde. Meanwhile, sake supplementation can rescue the brain damage caused by this toxic metal, resulting in a reduction of malondialdehyde and α-synuclein protein levels, plus a considerable improvement in blood serum total antioxidant capacity consideration (P<0.05). Activity behavioral tests confirmed the AS-mentioned changes by increasing the number of rotations and rod test time. Histopathology assays mimic the above data.
Conclusion: In sum, the sake yeast supplement due to its properties positively influences for improvement of dopaminergic neuron dysfunction via AS damage.
Keywords
Main Subjects
)