Document Type : Original Article
Authors
- Sadaf Saeedi 1
- Ali Olfati 2
- Tayebeh Sadeghi 3
- Firoozeh Veisi 4
- Maryam Zanganeh 4
- Nasrin Jalilian 4
- Sareh Farshadfar 5
- Samad Nazemi 6
- Hamed Khorami 7
1 Department of Pathobiology, Faculty of veterinary sciences, science and Research branch, Islamic Azad university, Tehran, Iran
2 Clinical Research Development Center, Motazedi Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran
3 Department of Physiology, Faculty of Medicine, Kerman Branch, Islamic Azad University, Kerman, Iran
4 Department Obstetrics & Gynecology, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran
5 Department of Pediatrics, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
6 Cellular and Molecular Research Center, Department of Physiology and Pharmacology, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
7 Department of Medical Chemistry, Ghadr University of Kuchesfahan, Gilan, Iran
Abstract
Background: Arsenic (AS) is widely distributed in our surroundings, causing various health problems like neurological disorders. The current research was designed to investigate the effect of the anti-oxidant and anti-inflammatory content of sake yeast on the recovery of brain damage in an AS-treated rat's model with behavioral, oxidative stress, and immunogenetics assessment.
Method: Twenty-four male rats were treated with AS (3 mg/kg b.wt. per day) alone or in combination form with sake (45 mg/kg b.wt. per day), and animals received them for 30 days in drinking water (n=6/group). The initial mechanism of action was explored by behavioral tests (rotarod, amphetamine rotation, and spatial memory(, oxidative assay, and histopathology methods.
Results: Considering the vehicle group, induction of brain abnormalities by AS significantly (P<0.05) decreased the number of substantia nigra neurons, total antioxidant capacity, glutathione peroxidase activity and increased the amount of α-synuclein protein and led to the massive accumulation of malondialdehyde. Meanwhile, sake supplementation can rescue the brain damage caused by this toxic metal, resulting in a reduction of malondialdehyde and α-synuclein protein levels, plus a considerable improvement in blood serum total antioxidant capacity consideration (P<0.05). Activity behavioral tests confirmed the AS-mentioned changes by increasing the number of rotations and rod test time. Histopathology assays mimic the above data.
Conclusion: In sum, the sake yeast supplement due to its properties positively influences for improvement of dopaminergic neuron dysfunction via AS damage.
Keywords
Main Subjects
- Verma CP, Bhatia R, Pandit V, Ashawat V. Arsenic induced diseases in human beings, their diagnosis & treatment. J Chem Pharm Re 2016;8(1):13-22.
- Dintsi BS, Letsoalo MR,Ambushe AA.Bioaccumulation and Human Health Risk Assessment of Arsenic and Chromium Species in Water–Soil–Vegetables System in Lephalale, Limpopo Province, South Africa. Mineral 2023; 13(7):930.
- Thakur M, Rachamalla M, Niyogi S, Datusalia AK, Flora SJS. Molecular Mechanism of Arsenic-Induced Neurotoxicity including Neuronal Dysfunctions. Int J Mol Sci. 2021; 22(18):10077.
- Rahman MA, Hannan MA, Uddin MJ, Rahman MS, Rashid MM, Kim B. Exposure to Environmental Arsenic and Emerging Risk of Alzheimer's Disease: Perspective Mechanisms, Management Strategy, and Future Directions. Toxics. 2021; 9(8):188.
- Davoodi M, Karimooy FN, Budde T, Ortega-Martinez S, Moradi-Kor N. Beneficial effects of Japanese sake yeast supplement on biochemical, antioxidant, and anti-inflammatory factors in streptozotocin-induced diabetic rats. Diabetes Metab Syndr Obes. 2019; 12:1667-1673.
- Vaghari M, Moghaddam AH, Ranjbar M, Moradikor N. Protective effects of Japanese sake yeast on depressive-like behaviors, oxidative stress and inflammatory parameters in a rat model of global cerebral ischemia/reperfusion. Biochem Biophys Res Commun. 2023; 674:97-101.
- Bozorgi H, Rashidy-Pour A, Moradikor N, Zamani M, Motaghi E. Neurobehavioral protective effects of Japanese sake yeast supplement against chronic stress-induced anxiety and depression-like symptoms in mice: Possible role of central adenosine receptors. Psychopharmacol (Berl). 2024; 241(2):401-416.
- Olfati A, Tvrda E. Riboflavin recovery of spermatogenic dysfunction via a dual inhibition of oxidative changes and regulation of the PINK1-mediated pathway in arsenic-injured rat model. Physiol Res. 2021; 70(4):591-603.
- Deacon RM. Measuring motor coordination in mice. J Vis Exp. 2013; 29;(75):e2609.
- Björklund A, Dunnett SB. The Amphetamine Induced Rotation Test: A Re-Assessment of Its Use as a Tool to Monitor Motor Impairment and Functional Recovery in Rodent Models of Parkinson's Disease. J Parkinsons Dis. 2019; 9(1):17-29.
- Nouri P, Olfati A. The Effects of Maternal Exposure Riboflavin to Prevent Uterus Arsenic Damage in Offspring Rats. J Hum Environ Health Promot. 2021; 7(4):182-8.
- Sokouti H, Mohajeri D, Nourazar MA. 6-Hydroxydopamine-Induced Neurotoxicity in Rat Model of Parkinson's Disease: Is Reversed via Anti-Oxidative Activities of Curcumin and Aerobic Exercise Therapy. Physiol Res. 2021; 71(4):551-560.
- Giralt A, Saavedra A, Carretón O, Xifró X, Alberch J, Pérez-Navarro E. Increased PKA signaling disrupts recognition memory and spatial memory: role in Huntington's disease. Hum Mol Genet. 2011; 20(21):4232-47.
- Erel O.A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004; 37(4):277-85.
- Medda N, Patra R, Ghosh TK,Maiti S. Neurotoxic Mechanism of Arsenic: Synergistic Effect of Mitochondrial Instability, Oxidative Stress, and Hormonal-Neurotransmitter Impairment. Biol Trace Elem Res. 2020; 198(1):8-15.
- Zhang C, Li Y, Yu H, Ye L, Li T, Zhang X, et al. Nanoplastics promote arsenic-induced ROS accumulation, mitochondrial damage and disturbances in neurotransmitter metabolism of zebrafish (Danio rerio). Sci Total Environ. 2023; 863:161005.
- Romano AD, Serviddio G, de Matthaeis A, Bellanti F, Vendemiale G. Oxidative stress and aging. J Nephrol 23 Suppl. 2010; 15:S29-36.
- Bustaffa E, Stoccoro A, Bianchi F, Migliore L. Genotoxic and epigenetic mechanisms in arsenic carcinogenicity. Arch Toxicol. 2014 88(5):1043-67.
- Babatunde Ilesanmi O, Roberts Inala E, Abiodun Lawal R. Neuroprotective Effect of Ethylacetate Fraction of Antiaris Africana against Sodium Azide-Induced Neurotoxicity in the Striata of Male Wistar Rats. Iran J Toxicol. 2023; 17(4):1-8.
- Yousuf R, Verma PK, Sharma P, Sood S, Bhatti MA, Bhat ZF. Neuroprotective effect of quercetin and Zingiber officinale on sodium arsenate-induced neurotoxicity in rats. Food Sci Nutr. 2023; 11(6):2964-2973.
- Kanungo J, Twaddle NC, Silva C,Robinson B, Wolle M, Conklin S. Inorganic arsenic alters the development of dopaminergic neurons but not serotonergic neurons and induces motor neuron development via Sonic hedgehog pathway in zebrafish. Neurosci Lett. 2023; 795:137042.
- Monoi N, Matsuno A, Nagamori Y, Kimura E, Nakamura Y, Oka K, et al. Japanese sake yeast supplementation improves the quality of sleep: a double-blind randomised controlled clinical trial. J Sleep Res .2016;25(1):116-23.
- Strandwitz P. Neurotransmitter modulation by the gut microbiota. Brain Res. 2016; 1693(Pt B):128-133.
- Franco R, Reyes-Resina I, Navarro G. Dopamine in Health and Disease: Much More Than a Neurotransmitter. Biomedicines. 2021; 9(2):109.
- Anguera JA, Reuter-Lorenz PA, Willingham DT, Seidler RD. Failure to engage spatial working memory contributes to age-related declines in visuomotor learning. J Cogn Neurosci. 2011; 23(1):11-25.
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