Document Type : Original Article
Authors
- Buddhika Wickramasingha 1
- Nirmani Jayasinghe 2
- Tania Warnakulasuriya 2
- Kushan Medagoda 2
- Wasanthi Subasinghe 1, 3
1 Centre for Sustainability Solutions, University of Kelaniya, Dalugama, Kelaniya, Sri Lanka
2 Department of Physiology, Faculty of Medicine, University of Kelaniya, Thalagolla Road, Ragama, Sri Lanka
3 Department of Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka
Abstract
Background: Gas station attendants (GSA) are at risk of adverse health outcomes due to chronic occupational exposure to petroleum hydrocarbons and poor occupational safety practices. In Sri Lanka, extended working hours, minimal use of personal protective equipment (PPE), and limited health surveillance exacerbate these risks. This study aimed to evaluate oxidative stress biomarkers and associated health risks among GSA in the Gampaha District.
Methods: A cross-sectional study was conducted among 25 exposed workers and 25 age-matched unexposed controls. Data on sociodemographic characteristics, occupational exposure history, lifestyle factors, and self-reported health symptoms were collected using a structured questionnaire. Blood samples were analyzed for oxidative stress biomarkers, including glutathione (GSH), 8-hydroxydeoxyguanosine (8-OHdG), and total antioxidant capacity (TAC), using enzyme-linked immunosorbent assay (ELISA).
Results: GSH levels were significantly higher among GSA (p = 0.043), indicating a potential early-phase compensatory antioxidant response to chronic petroleum vapor exposure. However, no statistically significant differences were found in TAC (p = 0.101) or 8-OHdG (p = 0.770) between exposed and control groups. Self-reported symptoms such as headaches, fatigue, memory disturbances, and respiratory complaints were more prevalent among the GSA. Alarmingly, PPE usage was extremely limited, with only 4% reporting access to masks and none to gloves, indicating a critical gap in occupational health protection.
Conclusion: The findings indicate early biochemical signs of oxidative stress among gas station attendees, alongside poor adherence to occupational safety practices. Immediate implementation of regulatory interventions, including mandatory PPE provision and health education, is essential to reduce long-term health risks in this vulnerable workforce.
Keywords
- 8-Hydroxy-2-deoxyguanosine (8-OHdG)
- Gas station workers
- Occupational exposure
- Oxidative stress
- Personal protective equipment
Main Subjects
- Çelik A, Çavaş T, Ergene‐Gözükara S. Cytogenetic biomonitoring in petrol station attendants: micronucleus test in exfoliated buccal cells. Mutagenesis. 2003;18(5):417-21.
- Thomas JS, Mercy P, Joseph M, Joseph B. Awareness, prevalence and factors associated with respiratory morbidities among selected petrol pump workers in Bengaluru City? Indian J Occup Environ Med. 2020; 24(3):199-202.
- Beerappa R, Venugopal D, Sen S, Ambikapathy M, Rao RHR. Assessment of 8-oxo-7, 8-dihydro-2′-deoxyguanosine as a marker of oxidative DNA damage in gasoline filling station attendants. International Journal of Occupational Medicine and Environmental Health. 2013;26:780-9.
- Etemadi A, Poustchi H, Chang CM, Calafat AM, Blount BC, Bhandari D, et al. Exposure to polycyclic aromatic hydrocarbons, volatile organic compounds, and tobacco-specific nitrosamines and incidence of esophageal cancer. J Natl Cancer Inst. 2024;116(3):379-88.
- Peters CE, Parent M-É, Harris SA, Bogaert L, Latifovic L, Kachuri L, et al. Occupational exposure to diesel and gasoline engine exhausts and the risk of kidney cancer in Canadian men. Ann Work Expo Health. 2018;62(8):978-89.
- Kachuri L, Villeneuve PJ, Parent M-É, Johnson KC, Group CCRER, Harris SA. Workplace exposure to diesel and gasoline engine exhausts and the risk of colorectal cancer in Canadian men. Environ Health. 2016;15:1-12.
- Scheepers PT, de Werdt L, van Dael M, Anzion R, Vanoirbeek J, Duca RC, et al. Assessment of exposure of gas station attendants in Sri Lanka to benzene, toluene and xylenes. Environ Res. 2019;178:108670.
- Uzma N, Kumar BS, Hazari MAH. Exposure to benzene induces oxidative stress, alters the immune response and expression of p53 in gasoline filling workers. Am J Ind Med. 2010;53(12):1264-70.
- Betteridge DJ. What is oxidative stress? Metabolism. 2000;49(2):3-8.
- Wu LL, Chiou C-C, Chang P-Y, Wu JT. Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics. Clin Chim Acta. 2004; 339(1-2):1-9.
- Deponte M. Glutathione catalysis and the reaction mechanisms of glutathione-dependent enzymes. Biochim Biophys Acta Gen Subj. 2013;1830(5):3217-66.
- Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative stress: harms and benefits for human health. Oxid Med Cell Longev. 2017; 2017(1):8416763.
- Muniz JF. Biomarkers of oxidative stress and DNA damage in agricultural workers. Oregon State University; 2010.
- Azeez OM, Akhigbe RE, Anigbogu CN. Exposure to petroleum hydrocarbon: implications in lung lipid peroxidation and antioxidant defense system in rat. Toxicol Int. 2012; 19(3):306.
- Yasin, SA, Salih ZR. Oxidative Stress Biomarkers in Gasoline Station Workers Chronically Exposed to Heavy Metals in Erbil City. J Appl Toxicol, 2025; 45(11): 2400-11.
- Elkama A, İlik N, Şentürk K, Karahalil B. Biomonitoring cytogenetic and oxidative-stress related damage in gas station attendants: Buccal micronucleus cytome assay and serum 8-OHdG levels. Arch Environ Occup Health, 2025; 80(7-8):165-73.
- Maksoud HA, Elharrif MG, Mahfouz M, Omnia M, Abdullah M, Eltabey M. Biochemical study on occupational inhalation of benzene vapours in petrol station. Respir Med Case Rep. 2019; 27:100836.
- Emara AM, El-Bahrawy H. Green tea attenuates benzene-induced oxidative stress in pump workers. J Immunotoxicol. 2008;5(1):69-80.
- MALINI SSN, Maithily K. Analysis of Oxidative Stress in Chronic Exposure to Petroleum Hydrocarbons in Karnataka, India. Asia Pac J Med Toxicol. 2017;6(1):6-11.
- Gupta N, Vyas S, Sankhla M, Punjabi P. Biochemical assessment of the hepatic functions of the petrol pump workers of Jaipur city. Natl J Physiol Pharm Pharmacol. 2017;7(10):1099-1099.
- Peterson CM, Thomas DM, Blackburn GL, Heymsfield SB. Universal equation for estimating ideal body weight and body weight at any BMI. Am J Clin Nutr. 2016;103(5):1197-203.
- Yung M, Dale AM, Kapellusch J, Bao S, Harris-Adamson C, Meyers AR, et al. Modeling the effect of the 2018 revised ACGIH® hand activity threshold limit value®(TLV) at reducing risk for carpal tunnel syndrome. J Occup Environ Hyg. 2019;16(9):628-33.
- Bahadar H, Mostafalou S, Abdollahi M. Current understandings and perspectives on non-cancer health effects of benzene: a global concern. Toxicol Appl Pharmacol, 2014; 276(2):83-94.
- Moro AM, Sauer E, Brucker N, Charão MF, Gauer B, Nunes do Nascimento S, et al. Evaluation of immunological, inflammatory, and oxidative stress biomarkers in gasoline station attendants. BMC Pharmacol Toxicol. 2019; 20:1-9.
- Prokopowicz A, Sobczak A, Szuła-Chraplewska M, Zaciera M, Kurek J, Szołtysek-Bołdys I. Effect of occupational exposure to lead on new risk factors for cardiovascular diseases. Occupational and environmental medicine. 2017; 74(5):366-73.
- Fenga C, Gangemi S, Teodoro M, Rapisarda V, Golokhvast K, Docea AO, et al. 8-Hydroxydeoxyguanosine as a biomarker of oxidative DNA damage in workers exposed to low-dose benzene. Toxicol rep. 2017; 4:291-5.
- Sule RO, Rivera GDT, Vaidya T, Gartrell E, Gomes AV. Environmental Toxins and Oxidative Stress: The Link to Cardiovascular Diseases. Antioxidants. 2025; 14(5):604.
- Hall CJ, Boyle RH, Astin JW, Flores MV, Oehlers SH, Sanderson LE, et al. Immunoresponsive gene 1 augments bactericidal activity of macrophage-lineage cells by regulating β-oxidation-dependent mitochondrial ROS production. Cell Metab. 2013; 18(2):265-78.
- Khan FH, Ambreen K, Fatima G, Kumar S. Assessment of health risks with reference to oxidative stress and DNA damage in chromium exposed population. Sci Total Environ. 2012; 430:68-74.
- Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J. 2012; 5:9-19.
- Malakootian M, Maleki S, Rajabi S, Hasanzadeh F, Nasiri A, Mohammadi A, et al. Source identification, spatial distribution and ozone formation potential of benzene, toluene, ethylbenzene, and xylene (BTEX) emissions in Zarand, an industrial city of southeastern Iran. Journal of Air Pollution and Health. 2022; 7(3):217-32.
- Laffon B, Fraga-Iriso R, Pérez-Cadahía B, Méndez J. Genotoxicity associated to exposure to Prestige oil during autopsies and cleaning of oil-contaminated birds. Food Chem Toxicol. 2006;44(10):1714-23.
- Laffon B, Pásaro E, Valdiglesias V. Effects of exposure to oil spills on human health: Updated review. J Toxicol Environ Health, Part B. 2016; 19(3-4):105-28.
- Bolden AL, Kwiatkowski CF, Colborn T. New look at BTEX: are ambient levels a problem? Environ Sci Technol. 2015;49(9):5261-76.
- Jayasinghe A, Priyanath H, Rathnayaka I, Premaratna S. Determinants and Challenges of Social Security for Tea Estate Workers: A Systematic Literature Review. DoE-UoC Working Paper 02. WP/DoE-UoC/2025/02, Department of Economics, University of Colombo, Sri Lanka.
- Davoudi-Kiakalayeh A, Mohammadi R, Pourfathollah AA, Siery Z, Davoudi-Kiakalayeh S. Alloimmunization in thalassemia patients: New insight for healthcare. Int J Prev Med. 2017;8(1):101.
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