Document Type : Original Article
Authors
- Mahmoud Ebrahimi 1
- Mohsen Ebrahimi 2
- Abolghasem Allahyari 3
- Mostafa Gholizadeh 4
- Mohammad Karimi 5
- Mohammad Reza Kazemi 6
- Marziyeh Karimi 7
1 Atherosclerosis Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
2 Department of Emergency Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3 Department of Clinical Hematology-Oncology, Mashhad University of Medical Sciences, Mashhad, Iran
4 Department of Chemistry, Faculty of Basic Sciences, Ferdowsi University, Mashhad, Iran
5 Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
6 Imam Reza Teaching Hospital Medical Center, Mashhad University of Medical Sciences, Mashhad, Iran
7 Faculty of Nursing, Islamic Azad University, Sabzevar Branch, Sabzevar, Iran
Abstract
Background: The pathogenesis of ethanol in the liver and kidney is associated with an increase in free radicals and oxidative stress that leads to structural and functional abnormalities in these vital organs.
Methods: Forty-eight male wistar rats were placed in eight groups and were treated as follows: control group (normal saline), group of ethanol (4 g/ kg), three doses of Ardeh and honey combination (1, 2 and 5 g/ kg body weight), and the three groups received three doses of Ardeh and honey combination with ethanol (the same three doses of combinations along with 4 g/ kg ethanol). All treatments were performed once daily for 60 consecutive days with method of stomach gavages. Serum, liver and kidney tissue samples were obtained in order to assess serum components, tissue antioxidant enzyme activities, and the level of thiobarbituric acid reactive substances (TBARS).
Results: Hepatic superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzymes in the ethanol group significantly decreased compared with the control group, while the Ardeh and honey combination (5 g) could only significantly increased glutathione peroxidase activity. Renal GPx activity in ethanol consumption significantly increased compared to controls. GPx activity in Ardeh and honey combination and alcohol (5 g, 4 g) were significantly reduced compared to ethanol group. In the present study, the amount of TBARS which represents the rate of lipid peroxidation, in liver of ethanol group compared with the control group showed a significant increase (P<0.05).
Conclusion: Ardeh and honey combination as a pre-treatment, protect liver and kidney against oxidative damage induced by ethanol.
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