Document Type : Original Article

Authors

1 Assistant Professor of Toxicology & Pharmacology, Medicinal plants Department, Imam Khomeini Higher Education Center, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. (Institute of Technical and Vocational Higher Education, Agriculture Jihad, AREEO, Tehran, Iran)

2 Laboratory Sciences Expert, Farabi Hospital Laboratory, Medical Sciences of Tehran University, Tehran, Iran

3 Associate Professor Ophthalmology, Emergency Ward, Farabi Hospital, Medical Sciences of Tehran University, Tehran, Iran

Abstract

Background: The fungi include microorganisms which are inhabitant in our environment and sometimes their produced poisons can result in Keratitis and if this disease is not treated appropriately, then blindness will occur. In the present study, the most infectious fungal agents which caused corneal ulcer in patients who referred to Farabi Eye Hospital of Tehran University of Medical Sciences have been identified. 
Methods: The sample was taken by ophthalmologist and then analyzed for identification of any fungal pathogen in accordance with laboratory standards. 
Results: Most fungi obtained from these samples were related to the genera Fusarium (n=60, 49.59±2.96%), Aspergillus (n=32, 
26.44±2.52%), Candida albicans (n=17, 14.05±2.32%) and other fungi (n=12, 9.92±3.16%), respectively. Fungal Keratitis (FK) was observed mostly in men (n=96, 79.34±0.52%). Whilst for women, FK rate was found to be (n= 25, 20.66±0.52). Our results showed that in farmers and constructional workers, due to trauma there was seen an increased number of FK which resulted from hitting the plant organs and objects in eyes.
Conclusion: It can be concluded that after corneal damage by these fungal agents, toxic metabolite is emerged and finally the human eye will be hurt.
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Keywords

 
1. Karlovsky P. Biological detoxification of the mycotoxin deoxynivalenol and its use in genetically engineered crops and feed additives. Appl Microbiol Biotechnol 2011; 91: 491–504.
2. Balali GR, Minaefar A, Sharifnabi B. Zymography variation of pectinase in Aspergillus niger and Aspergillus flavus. Iran J Biol 2007; 20:5-14.
3. Ghazani MH. Aflatoxin M1 contamination in pasteurized milk in Tabriz (northwest of Iran). Food Chem Toxicol 2009; 
47:1624-5. 
4. Mravičić I, Dekaris I, Gabrić N, Romac I, Glavota V, Mlinarić-Sviben M. Trichophyton Spp. fungal keratitis in 22 years old female contact lenses wearer. Coll Antropol 2010; 34: 271–4.
5. Ledbetter EC, Patten VH, Scarlett JM, Vermeylen FM. In vitro susceptibility patterns of fungi associated with keratomycosis in horses of the northeastern United States: 68 cases (1987-2006). J Am Vet Med Assoc 2007; 231: 1086-91.
6. Srinivasan M. Fungal keratitis. Curr Opin ophthalmol 2004; 15:321-7.
7. Gelatt KN. Essentials of Veterinary Ophthalmology: Canine Corneal: Diseases and Surgery. 2nd ed.United States: Wiley-Blackwell Pub; 2008.
8. Whitcher JP, Srinivasan M, Upadhyay MP. Corneal blindness: A global perspective. Bull World Health Organ 2001; 79:214–21.
9. De Lucca AJ. Harmful fungi in both agriculture and medicine. Rev Iberoam Micol 2007; 24: 3-13.
10. Anaissie EJ, Kuchar RT, Rex JH, Francesconi A, Kasai M, Müller FM et al. Fusariosis associated with pathogenic 
Fusarium species colonization of a hospital water system: a new paradigm for the epidemiology of opportunistic mold 
infections. Clin Infect Dis 2001; 33:1871-8.
11. Krysinska-Traczyk E, Kiecana I, Perkowski J, Dutiewicz J. Levels of fungi and mycotoxins in samples of grain and grain dust collected on farms in Eastern Poland. Ann Agric Environ Med 2001; 8: 269-74.
12. Bharathi MJ, Ramakrishnan R, Meenakshi R, Padmavathy S, Shivakumar C, Srinivasan M. Microbial keratitis in South 
India: influence of risk factors, climate, and geographical variation. Ophthalmic Epidemiol 2007; 14:61–9.
13. Xie L, Zhong W, Shi W, Sun S. Spectrum of fungal keratitis in north China. Ophthalmology 2006; 113:1943–8.
14. Alfonso EC, Cantu-Dibildox J, Munir WM, Miller D, O'Brien TP, Karp CL et al. Insurgence of Fusarium keratitis associated with contact lens wear. Arch Ophthalmol 2006; 124:941–7.
15. Khor WB, Aung T, Saw SM, Wong TY, Tambyah PA, Tan AL et al. An outbreak of Fusarium keratitis associated with contact lens wear in Singapore. JAMA 2006; 295: 2867–73.
16. Gaujoux T, Chatel MA, Chaumeil C, Laroche L, Borderie VM. Outbreak of contact lens-related Fusarium keratitis in France. Cornea 2008; 27:1018–21.
17. Ma SK, So K, Chung PH, Tsang HF, Chuang SK. A multi-country outbreak of fungal keratitis associated with a brand of contact lens solution: the Hong Kong experience. Int J Infect Dis 2009; 13:443–8.
18. Thomas PA, Kaliamurthy J. Mycotic keratitis: epidemiology,diagnosis and management. Clin Microbiol Infect 2013; 
19:210–20.
19. Ebadollahinatanzi A, Arabrahmatipour G, Tabatabaei SA. Identification of the most common pathogens formed by 
environmental fungus toxins in human`s eye. Toxicol Lett 2014; 229: S132-3.
20. Balloy V, Huerre M, Latgé JP, Chignard M. Differences in patterns of infection and inflammation for corticosteroid 
treatment and chemotherapy in experimental invasive pulmonary aspergillosis. Infect Immun 2005; 73: 494-503.
21. Bindu N. Fungal Keratitis. Kerala J Ophthalmol 2008; 20:169-73.
22. Richardson MD, Warnock DW. Fungal infection: diagnosis and management, 4th ed. England; Wiley-Blackwell: 2012.
23. Dóczi I, Gyetvai T, Kredics L, Nagy E. Involvement of Fusarium spp. in fungal keratitis. Clin Microbiol Infect 2004; 
10: 773-6.